BIOSYNTHETIC FUNCTIONAL GENE ANALYSIS OF BIS-INDOLE METABOLITES FROM 25D7, A CLONE DERIVED FROM A DEEP-SEA SEDIMENT METAGENOMIC LIBRARY

Biosynthetic Functional Gene Analysis of Bis-Indole Metabolites from 25D7, a Clone Derived from a Deep-Sea Sediment Metagenomic Library

This work investigated the metabolites and their biosynthetic functional hydroxylase genes of the deep-sea sediment metagenomic clone 25D7.5-Bromoindole was added to the 25D7 clone derived Escherichia coli fermentation broth.The new-generated metabolites and their biosynthetic byproducts were located through LC-MS, in which the isotope peaks of bro

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A bacterial binary toxin system that kills both insects and aquatic crustaceans: Photorhabdus insect-related toxins A and B.

Photorhabdus insect-related toxins A and B (PirA and PirB) were first recognized as insecticidal toxins from Photorhabdus luminescens.However, subsequent studies showed that their homologs from Vibrio parahaemolyticus also play critical roles in the pathogenesis of acute hepatopancreatic necrosis disease (AHPND) in shrimps.Based on the structural f

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The Expression Pattern of Prostaglandin-Endoperoxide Synthase-2 in Immature Oocytes and Surrounding Cumulus Cells May Explain A Disrupted Oocyte Maturation Process

Objective: To evaluate the correlation between the gene expression of prostaglandin-endoperoxide synthase-2 in the cumulus-oocyte complex with the level of prostaglandin-endoperoxide synthase-2 in follicular fluid and follicle diameter, oocyte maturation, and fertilization capacity.Study Design: As the study group, 26 cumulus-oocyte complexes and 2

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